The Historic Brownsville Museum is located at the old Southern Pacific Railroad Depot. Built in 1928, the Southern Pacific Railroad Depot is recognized as a Texas Historical Landmark and is listed on the National Register of Historic Places. Inside the depot, the museum details the events that led to the establishment of Brownsville and displays temporary exhibits that highlight various topics related to local history.

Also included in your admission to the museum is the Simon Celaya building, and the Mary A. Yturria Education Center.